Physical methods in clinical chemistry
Abbreviation: 46LA2 Load: 15(L) + 0(S) + 0(CP) + 0(EXE) + 0(P) + 0(ST) + 45(LE) + 0(ME) + 0(SCP) + 0(FE) + 0(CE) + 0(PP) + 0(MSCP)
Lecturers in charge: dr. sc. Jasna Leniček Krleža
Course description: Objectives of the course are:
- to acquire basic knowledge of physical methods used in clinical chemistry
- master the performance techniques in the application of physical methods - manual or automatic analyzers
- Meet with state-of-the-art technology of medical biochemistry laboratories
Spectrophotometry and Spectrographics. Light Absorption Laws, Spectral Photometry, Spectrum Photometry Surveys, Indoor and Outdoor Photo Effects, Radiation Receptors, Display Results. Laws broadcast analysis. Nefelometry and turbidimetry. Optical Methods for Measuring Colloidal Colloidal Systems. Mass spectrometry. Mass spectrometer operation principle, mass spectra. Refractometry and polarimetry. Light fading laws, refractometric apparatus. Generally about polarization of light, polarimeter construction, polarimetric analysis. Fluorimetry. Fluorescence and phosphorescence, the physical mechanism of fluorescence. Fluorimeters with optical filters and spectrophotometers. Flame photometry. The theoretical basis of flame photometry, atomic emission of flame photometry and atomic absorption spectrophotometry. Theoretical basics of pH determination, colorimetric and potentiometric determination of pH. Ion selective electrodes and biosensors. Determination of hydrogen ion concentration. Lead, glass and calomel electrodes. Ion selective electrodes - membrane potential. Chromatography, theoretical basis of absorption and distribution chromatography. Column chromatography, plate and gas chromatography, HPLC. Electrophoresis, theoretical basis, electrophoresis on paper, thin layer and gel. Immunoelectrophoresis. Chemiluminescence and bioluminescence, theory and application in analytical chemistry. Basics of instrumental analytics in molecular biology. Automation in Clinical Chemistry. Discontinued and continuous automatic analyzers, single channel and multichannel self-analyzers. Application of DNA Analysis in Judicial Medicine.
Lecture languages: - - -
Compulsory literature:
1. R. K. Murray i sur.: Harperova ilustrirana biokemija, prijevod 28th izdanja; Medicinska naklada, Zagreb, 2011.
2. Peter Atkins, Julio De Paula: Physical Chemistry, Oxford University Press, 9th Ed., Oxford, 2010.
3. J. Lovrić i sur.: Priručnik za vježbe iz medicinske kemije i biokemije za studente medicine, Medicinska naklada,Zagreb 2009.
4. Štraus, B., Stavljenić-Rukavina, A., Plavšić, F.: Analitičke tehnike u kliničkom laboratoriju, Medicinska naklada, Zagreb, 1997., odabrana poglavlja
Recommended literature:
5. Tiez: Fundamentals of Clinical Chemistry, odabrana poglavlja
6. Rogić D, Stavljenić-Rukavina A.: Laboratorijska dijagnostika u trećem mileniju, Acta Medica Croatica, 55;59-60, 2002.
7. T. McKee, J. McKee: Biochemistry, McGrew-Hill, USA, 2006.; udžbenik
L - Lectures
FE - Field exercises
CE - Project laboratory
PP - Preclinical practicals
MSCP - Mentorship of special clinical practicals
S - Seminar
CP - Clinical practicals
EXE - Exercises of applied kinesiology
P - Practicum
ST - Sectio training
LE - Laboratory exercises
ME - Methodology exercises
SCP - Special clinical practicals
* - Not graded