Molecular biology
Abbreviation: 46LA2 Load: 15(L) + 15(S) + 0(CP) + 0(EXE) + 0(P) + 0(ST) + 30(LE) + 0(ME) + 0(SCP) + 0(FE) + 0(CE) + 0(PP) + 0(MSCP)
Lecturers in charge: dr. sc. Ivna Kocijan
Course description: Lectures will cover the most modern settings molecular biology and experimental
models of molecular biology research. The central dogma of molecular biology.
Mechanisms of DNA duplication. Replication forks. Okazaki fragments. DNA polymerase.
High reliability replication of the DNA molecule. Mechanisms of DNA repair. Effect
gene. RNA polymerase. Redrawing the RNA molecule. Reverse transcription. regulation
transcription in prokaryotes and eukaryotes. Genetics of bacteria, genetic engineering.
Recombinant DNA, restriction endonucleases, ligases. RFLP minisatelli, microsatellites,
SNP. Genomics. Proteomics. Genetic maps: physical and map-related genes. Molecular
base mutations. Point mutations. Mutagenesis and cancerogenesis. Gene Therapy,
transgenic animals, 0-mutants and cloning. Biotechnology. At seminars and training courses
will introduce the basic methods in molecular biology. Electrophoretic techniques and
which allow the separation and determination of specific nucleic acid fragments
and protein; work with experimental životinjama- isolation early stages of mammalian embryo,
microinjection of DNA into the oocyte.
Lecture languages: - - -
Compulsory literature:
1. Cooper G.M., Hausman R.E.: Stanica - molekularni pristup, Medicinska naklada, Zagreb, 2004. (odabrana poglavlja)
2. Ristov A. i sur.: Metode u molekularnoj biologiji. Institut Ruđer Bošković, Zagreb, 2007.
Recommended literature:
3. Pavlica M.: Mrežni udžbenik iz genetike.
L - Lectures
FE - Field exercises
CE - Project laboratory
PP - Preclinical practicals
MSCP - Mentorship of special clinical practicals
S - Seminar
CP - Clinical practicals
EXE - Exercises of applied kinesiology
P - Practicum
ST - Sectio training
LE - Laboratory exercises
ME - Methodology exercises
SCP - Special clinical practicals
* - Not graded