General clinical chemistry
Abbreviation: 46LA2 Load: 30(L) + 0(S) + 60(CP) + 0(EXE) + 0(P) + 0(ST) + 0(LE) + 0(ME) + 0(SCP) + 0(FE) + 0(CE) + 0(PP) + 0(MSCP)
Lecturers in charge: dr. sc. Jasna Leniček Krleža
Course description: Introduction to Clinical Chemistry: the history and development of the profession. The preanalytical phase. Analytical procedures in
clinical chemistry. Biological materials. Urinalysis: chemical, morphological; standard and
using flow cytometry. Introduction to enzymes, enzymes. Isoenzymes. The enzyme and isoenzyme
Indicators of liver disease. Creatine kinase and CK isoenzymes in the diagnosis of myocardial infarction.
Differences between the enzyme activity and for the mass of the enzyme. Enzymes and isoenzymes
typical for pancreatic disease. Non-protein nitrogen compounds. Creatinine. uric
acid. Creatinine clearance. Carbohydrates. Glucose. Lactose. Galactose. tests load
in the diagnosis of diabetes. Bilirubin. Gall colors and bile acid. Hemoglobin. Iron and biological
important inorganic compounds. Protein carriers of iron. Electrolytes. Trace elements.
Lipids: cholesterol, triacylglycerols, fatty acids. Lipoproteins: structure and methods
HDL, LDL and VLDL liporpoproteina, apoproteins. Risk and desirable lipid values and
lipoproteins. Introduction to proteins. Protein separation techniques. Immunoassays.
Immunoassays with labeled Ag / At. Lipids and lipoproteins. Protein in the urine? algorithm
diagnosis of kidney disease. The division of the laboratory according to the level of health care,
corresponding time. The necessary equipment, support services. Laboratory of Medical Biochemistry at
the level of primary healthcare facilities (emergency work under the ordinary laboratory work).
Specialized laboratories, the structure employed in clinical laboratories. intralaboratory
service organization to the specifics of that process. Principles of acceptance samples.
Optimization of the analytical phase. Post-stage? critical point of each laboratory.
Interpretive located. The responsibility of the medical staff in the process of laboratory
Search. Integration of laboratories in different health systems. consolidation and
centralization versus point-of-care testing? advantages and disadvantages.
Exercises: Preparation of reagents. Qualitative analysis of urine. Determination of calcium, inorganic
phosphorus, magnesium, copper, iron, bilirubin, total protein, urea, uric acid,
creatinine, glucose, total lipids, triglycerides, phospholipids, cholesterol, HDL and LDLkolesterola.
Electrophoretic separation of serum proteins and lipoproteins. Determination
activity of alpha - amylase, alkaline phosphatase, GGT, ALT / AST total activity of creatine kinase and
CK isoenzyme MB, LDH, alpha - HBDH.
Lecture languages: - - -
Compulsory literature:
1. Čvorišćec D, Čepelak I, ur. Štrausova medicinska biokemija. Medicinska naklada, Zagreb, 2009.
2. Tietz: Fundamentals of clinical chemistry. Saunders Elsevier, St. Louis, 2008.
3. Berg J. M, Tymoczko J. L, Stryer L. Biokemija, Školska knjiga, Zagreb, 2013.
4. Sertić J.i sur. Klinička kemija i molekularna dijagnostika u kliničkoj praksi. Medicinska naklada, Zagreb, 2015.
5. TM Devlin: Texbook of Biochemistry with Clinical Correlation, J. Wiley & sons, New York, 2011.
Recommended literature:
6. Tiez: Fundamentals of Clinical Chemistry, odabrana poglavlja
L - Lectures
FE - Field exercises
CE - Project laboratory
PP - Preclinical practicals
MSCP - Mentorship of special clinical practicals
S - Seminar
CP - Clinical practicals
EXE - Exercises of applied kinesiology
P - Practicum
ST - Sectio training
LE - Laboratory exercises
ME - Methodology exercises
SCP - Special clinical practicals
* - Not graded