| Methods of growing cells in vitro |
| Abbreviation: 46LA03
|
Load: 15(L)
+ 0(S)
+ 15(CP)
+ 0(EXE)
+ 0(P)
+ 0(ST)
+ 0(LE)
+ 0(ME)
+ 0(SCP)
+ 0(FE)
+ 0(CE)
+ 0(PP)
+ 0(MSCP)
|
| Lecturers in charge: |
dr. sc. Mirna Golemović |
| Lecturers: |
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| Course description: Spatial organization of the in vitro cell culture laboratory, operation under sterile conditions and use and maintenance of
appropriate laboratory equipment, cell bases in vitro, preparation of cell suspensions from various tissue sources, isolation
techniques of individual cell populations, basic biological terms related to different types of stem cells , basal concepts
related to cellular therapy, baseline baseline cellular baseline and its clinical application, basis of the mesenchymal stem
cell and its clinical application, the importance of functional tests in clinical use of cells cultured in vitro.
|
| Compulsory literature: |
| 1. |
Batinić D, Petrovečki M, Golemović M. Uzgoj krvotvornih stanica in vitro. U: Batinić D. (editor): Metode istraživanja in vitro
i in vivo. Zagreb: Medicinska naklada; 2004:69-76.
|
| Recommended literature: |
| 2. |
Lourdes M. Caselli-Fernandez, Robert Terkola. Clean room environment, personnel, quality assurance and their monitoring. EJHPPractice.
2006;12:29-34.
|
| 3. |
HG Drexler, WG Dirks, Y Matsuo and RAF MacLeod. False leukemia-lymphoma cell lines: an update on over 500 cell lines. Leukemia
2003;17:416-42.
|
| 4. |
Bieback K, Kern S, Klüter H, Eichler H. Critical parameters for the isolation of mesenchymal stem cells from umbilical cord
blood. Stem Cells. 2004;22(4):625-34.
|
| 5. |
Ivanovic Z, Duchez P, Dazey B, Hermitte F, Lamrissi-Garcia I, Mazurier F, Praloran V, Reiffers J, Vezon G, Boiron JM.A clinical-scale
expansion of mobilized CD 34+ hematopoietic stem and progenitor cells by use of a new serum-free medium. Transfusion. 2006
Jan;46(1):126-31.
|
| 6. |
Tung SS, Parmar S, Robinson SN, De Lima M, Shpall EJ. Ex vivo expansion of umbilical cord blood for transplantation. Best
Pract Res Clin Haematol. 2010 Jun;23(2):245-57.
|
| 7. |
McKenna D, Sheth J. Umbilical cord blood: current status & promise for the future. Indian J Med Res. 2011 Sep;134:261-9. |
| 8. |
Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F, Krause D, Deans R, Keating A, Prockop Dj, Horwitz E. Minimal
criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement.
Cytotherapy. 2006;8(4):315-7.
|
| 9. |
Fossett E, Khan WS. Optimising human mesenchymal stem cell numbers for clinical application: a literature review. Stem Cells
Int. 2012;2012:465259.
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| 10. |
Benderitter M, Gourmelon P, Bey E, Chapel A, Clairand I, Prat M, Lataillade JJ. New emerging concepts in the medical management
of local radiation injury. Health Phys. 2010 Jun;98(6):851-7.
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