| Electrophoretic and chromatographic techniques in lipid and lipoprotein analysis |
| Abbreviation: 46LA03
|
Load: 15(L)
+ 0(S)
+ 15(CP)
+ 0(EXE)
+ 0(P)
+ 0(ST)
+ 0(LE)
+ 0(ME)
+ 0(SCP)
+ 0(FE)
+ 0(PL)
+ 0(PCP)
+ 0(MSCP)
|
| Lecturers in charge: |
Adriana Unić MMedBiochem |
| Lecturers: |
|
Course description: Emphasis is placed on laboratory work which will give students the knowledge and skills
basic techniques of isolation,
separation and identification of lipid and lipoprotein physiological and pathological
states in biological samples. By
default theme with the help of stakeholders and collaborators Course
to analyze the biological sample, and the results
compared and discussed with the searched
literature
The aim of the course is to provide students with the knowledge
necessary for independent laboratory analysis in
lipid and lipoprotein biological samples with application in endocrinology,
the diagnosis of hereditary
diseases, immunology, physiology, molecular biology, genetics, pharmacogenetics and
cardiovascular
medicine. The course covers the practical part of the course of biological / biochemical
separation methods in the analysis
of lipids and lipoproteins, and includes a planned introduction, discussion principles
separation and purification of
biomolecules strategy and instrumental analysis techniques lipids and
lipoproteins.
Lipids il lipoproteins: structure
and site: Fatty acids; The most simple lipids and
lipoproteins and complex glycerophospholipids in the tissues; sphingolipids;
The composition of lipids and lipoproteins
animal tissues;
Extraction, storage and handling of specimens: Practical
Proposals (storage tissue
preliminary treatments prior to extraction, minimizing auto-oxidation, lipid storage
extract,
pollutants and noise during extraction, poisons); The principles of the extraction process
solvents; Preferred methods
of extraction (method by Folch-in method by Lees and Stanley,
Method according to Bligh and Dyer, special cases, chromatographic
analysis of lipids);
Chromatographic and electrophoretic analysis of lipids and lipoproteins: general principles: Mode
of separation
for chromatography; Gas chromatography and electrophoresis; High performance liquid chromatography
effectiveness;
Thin-layer chromatography; Supercritical fluid chromatography; mass
spectrometry;
Analysis of fatty acid: Preparation
of derivatives of fatty acids; Gas chromatographic analysis
fatty acid derivatives; Isolation and identification of fatty
acids by spectroscopic and chemical
degradable techniques;
Analysis of molecular species of lipids and lipoproteins:
Triacylglycerols, diacylglycerols derived from
complex and related lipids; Phospholipids and glycolipids; related lipids
and lipoproteins .
|
| Compulsory literature: |
| 1. |
Čvorišćec D, Čepelak I, ur. Štrausova medicinska biokemija. 3. izd. Zagreb: Medicinska naklada, 2009. |
| 2. |
Jadranka Sertić i sur. Klinička kemija i molekularna dijagnostika u kliničkoj praksi. 2 izd. Zagreb: Medicinska naklada, 2015. |
| Recommended literature: |
| 3. |
http://www.lipid.co.uk
|
| 4. |
http://www.cyberlipid.org
|
| 5. |
Lovrić J, Sertić J, ur. Harperova ilustrirana biokemija Zagreb: Medicinska naklada, 2011. |
| 6. |
Lovrić J, Sertić J, ur. Harperova ilustrirana biokemija Zagreb: Medicinska naklada, 2011. |
| 7. |
Čepelak I, i sur. Medicinsko.biokemijske smjernice; Medicinska naklada, Zagreb, 2004 |
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